The validation of the Z-Scan technique for the determination of plasma glucose

Sarah I. Alves; Elaine A. O. Silva; Simone S. Costa; Denise R. N. Sonego; Maira L. Hallack; Ornela L. Coppini; Fernanda Rowies; Ligia A. Azzalis; Virginia B. C. Junqueira; Edimar C. Pereira; Katya C. Rocha; Fernando L. A. Fonseca

doi:10.1117/12.2025994

18 November 2013 The validation of the Z-Scan technique for the determination of plasma glucose

Sarah I. Alves, Elaine A. O. Silva, Simone S. Costa, Denise R. N. Sonego, Maira L. Hallack, Ornela L. Coppini, Fernanda Rowies, Ligia A. Azzalis, Virginia B. C. Junqueira, Edimar C. Pereira, Katya C. Rocha, Fernando L. A. Fonseca

Author Affiliations +

Proceedings Volume 8785, 8th Iberoamerican Optics Meeting and 11th Latin American Meeting on Optics, Lasers, and Applications; 87851B (2013) https://doi.org/10.1117/12.2025994
Event: 8th Ibero American Optics Meeting/11th Latin American Meeting on Optics, Lasers, and Applications, 2013, Porto, Portugal

Abstract

Glucose is the main energy source for the human body. The concentration of blood glucose is regulated by several hormones including both antagonists: insulin and glucagon. The quantification of glucose in the blood is used for diagnosing metabolic disorders of carbohydrates, such as diabetes, idiopathic hypoglycemia and pancreatic diseases. Currently, the methodology used for this determination is the enzymatic colorimetric with spectrophotometric. This study aimed to validate the use of measurements of nonlinear optical properties of plasma glucose via the Z-Scan technique. For this we used samples of calibrator patterns that simulate commercial samples of patients (ELITech ©). Besides calibrators, serum glucose levels within acceptable reference values (normal control serum - Brazilian Society of Clinical Pathology and Laboratory Medicine) and also overestimated (pathological control serum - Brazilian Society of Clinical Pathology and Laboratory Medicine) were used in the methodology proposal. Calibrator dilutions were performed and determined by the Z-Scan technique for the preparation of calibration curve. In conclusion, Z-Scan method can be used to determinate glucose levels in biological samples with enzymatic colorimetric reaction and also to apply the same quality control parameters used in biochemistry clinical.

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Sarah I. Alves, Elaine A. O. Silva, Simone S. Costa, Denise R. N. Sonego, Maira L. Hallack, Ornela L. Coppini, Fernanda Rowies, Ligia A. Azzalis, Virginia B. C. Junqueira, Edimar C. Pereira, Katya C. Rocha, and Fernando L. A. Fonseca "The validation of the Z-Scan technique for the determination of plasma glucose", Proc. SPIE 8785, 8th Iberoamerican Optics Meeting and 11th Latin American Meeting on Optics, Lasers, and Applications, 87851B (18 November 2013); https://doi.org/10.1117/12.2025994

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