Optical clearing of human skin for the enhancement of optical imaging of proximal interphalangeal joints

Ekaterina A. Kolesnikova; Aleksandr S. Kolesnikov; Urszula Zabarylo; Olaf Minet; Elina A. Genina; Alexey N. Bashkatov; Valery V. Tuchin

doi:10.1117/12.2049525

30 January 2014 Optical clearing of human skin for the enhancement of optical imaging of proximal interphalangeal joints

Ekaterina A. Kolesnikova, Aleksandr S. Kolesnikov, Urszula Zabarylo, Olaf Minet, Elina A. Genina, Alexey N. Bashkatov, Valery V. Tuchin

Author Affiliations +

Proceedings Volume 9031, Saratov Fall Meeting 2013: Optical Technologies in Biophysics and Medicine XV; and Laser Physics and Photonics XV; 90310C (2014) https://doi.org/10.1117/12.2049525
Event: Saratov Fall Meeting 2013, 2013, Saratov, Russian Federation

Abstract

We are proposing a new method for enhancement of optical imaging of proximal interphalangeal (PIP) joints in humans at skin using optical clearing technique. A set of illuminating laser diodes with the wavelengths 670, 820, and 904 nm were used as a light source. The laser diodes, monochromatic digital CCD camera and specific software allowed for detection of the finger joint image in a transillumination mode. The experiments were carried out in vivo with human fingers. Dehydrated glycerol and hand cream with urea (5%) were used as optical clearing agents (OCAs). The contrast of the obtained images was analyzed to determine the effect of the OCA. It was found that glycerol application to the human skin during 60 min caused the decrease of contrast in 1.4 folds for 670 nm and the increase of contrast in 1.5 and 1.7 folds for 820 nm and 904 nm, respectively. At the same time, the hand cream application to the human skin during 60 min caused the decrease of contrast in 1.1 folds for 670 nm and the increase of contrast in 1.3 and 1.1 folds for 820 nm and 904 nm, respectively. The results have shown that glycerol and the hand cream with 5% urea allow for obtaining of more distinct image of finger joint in the NIR. Obtained data can be used for development of optical diagnostic methods of rheumatoid arthritis.

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Ekaterina A. Kolesnikova, Aleksandr S. Kolesnikov, Urszula Zabarylo, Olaf Minet, Elina A. Genina, Alexey N. Bashkatov, and Valery V. Tuchin "Optical clearing of human skin for the enhancement of optical imaging of proximal interphalangeal joints", Proc. SPIE 9031, Saratov Fall Meeting 2013: Optical Technologies in Biophysics and Medicine XV; and Laser Physics and Photonics XV, 90310C (30 January 2014); https://doi.org/10.1117/12.2049525

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