Fluorescence lifetime imaging microscopy using a streak camera

Lixin Liu; Yahui Li; Luogeng Sun; Heng Li; Xiao Peng; Junle Qu

doi:10.1117/12.2039056

28 February 2014 Fluorescence lifetime imaging microscopy using a streak camera

Lixin Liu, Yahui Li, Luogeng Sun, Heng Li, Xiao Peng, Junle Qu

Proceedings Volume 8948, Multiphoton Microscopy in the Biomedical Sciences XIV; 89482L (2014) https://doi.org/10.1117/12.2039056
Event: SPIE BiOS, 2014, San Francisco, California, United States

Abstract

We present the development of a fluorescence lifetime imaging microscopy system using a streak camera (SC-FLIM), which uses ultrafast infrared laser for multiphoton excitation and a streak camera for lifetime measurement. A pair of galvo mirrors are employed to accomplish quick time-resolved scanning on a line and 2D fluorescence lifetime imaging. The SC-FLIM system was calibrated using an F-P etalon and several standard fluorescent dyes, and was also used to perform fluorescence lifetime imaging of fluorescent microspheres and a prepared plant stem slide.

Citation Download Citation

Lixin Liu, Yahui Li, Luogeng Sun, Heng Li, Xiao Peng, and Junle Qu "Fluorescence lifetime imaging microscopy using a streak camera", Proc. SPIE 8948, Multiphoton Microscopy in the Biomedical Sciences XIV, 89482L (28 February 2014); https://doi.org/10.1117/12.2039056

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