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Proceedings Article

Intracellular temperature mapping with fluorescence-assisted photoacoustic thermometry

[+] Author Affiliations
Liang Gao, Chi Zhang, Chiye Li, Lihong Wang

Washington Univ. in St. Louis (United States)

Proc. SPIE 8943, Photons Plus Ultrasound: Imaging and Sensing 2014, 894309 (March 3, 2014); doi:10.1117/12.2038569
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From Conference Volume 8943

  • Photons Plus Ultrasound: Imaging and Sensing 2014
  • Alexander A. Oraevsky; Lihong V. Wang
  • San Francisco, California, United States | February 01, 2014

abstract

Measuring intracellular temperature is critical to understanding many cellular functions but still remains challenging. Here we present a technique – fluorescence-assisted photoacoustic thermometry (FAPT) – for intracellular temperature mapping applications. To demonstrate FAPT, we monitored the intracellular temperature distribution of HeLa cells with sub-degree (0.7 °C) temperature resolution and sub-micron (0.23 μm) spatial resolution at a sampling rate of 1 kHz. Compared to traditional fluorescence-based methods, FAPT features the unique capability of transforming a regular fluorescence probe into a concentration- and excitation-independent temperature sensor, bringing a large collection of commercially available generic fluorescent probes into the realm of intracellular temperature sensing. © (2014) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Citation

Liang Gao ; Chi Zhang ; Chiye Li and Lihong Wang
" Intracellular temperature mapping with fluorescence-assisted photoacoustic thermometry ", Proc. SPIE 8943, Photons Plus Ultrasound: Imaging and Sensing 2014, 894309 (March 3, 2014); doi:10.1117/12.2038569; http://dx.doi.org/10.1117/12.2038569


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