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Proceedings Article

Fluorescence spectroscopy using excitation and emission matrix for quantification of tissue native fluorophores and cancer diagnosis

[+] Author Affiliations
Binlin Wu

Weill Cornell Medical College (United States)

S. K. Gayen

The City College of New York, CUNY (United States)

M. Xu

Fairfield Univ. (United States)

Proc. SPIE 8926, Photonic Therapeutics and Diagnostics X, 89261M (March 4, 2014); doi:10.1117/12.2040985
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From Conference Volume 8926

  • Photonic Therapeutics and Diagnostics X
  • Bernard Choi; Nikiforos Kollias; Haishan Zeng; Hyun Wook Kang; Brian J. F. Wong; Justus F. Ilgner; Guillermo J. Tearney; Kenton W. Gregory; Laura Marcu; Andreas Mandelis; Michael D. Morris
  • San Francisco, California, United States | February 01, 2014

abstract

Native fluorescence spectrum of normal and cancerous human prostate tissues is studied to distinguish between normal and cancerous tissues, and cancerous tissues at different cancer grade. The tissue samples were obtained from Cooperative Human Tissue Network (CHTN) and National Disease Research Interchange(NDRI). An excitation and emission matrix (EEM) was generated for each tissue sample by acquiring native fluorescence spectrum of the sample using multiple excitation wavelengths. The non-negative matrix factorization algorithm was used to generate fluorescence EEMs that correspond to the fluorophores in biological tissues, including tryptophan, collagen, elastin, nicotinamide adenine dinucleotide (NADH), flavin adenine dinucleotide (FAD) and the background paraffin. We hypothesize that, as a consequence of metabolic changes associated with the development of cancer, the concentrations of NADH and FAD are different in normal and cancerous tissues, and also different for different cancer grades. We used the ratio of the abundances of FAD and NADH to distinguish between normal and cancerous tissues, and the tissue cancer grade. The FAD-to-NADH ratio was found to be the highest for normal tissue and decreased as the cancer grade increased. © (2014) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Citation

Binlin Wu ; S. K. Gayen and M. Xu
" Fluorescence spectroscopy using excitation and emission matrix for quantification of tissue native fluorophores and cancer diagnosis ", Proc. SPIE 8926, Photonic Therapeutics and Diagnostics X, 89261M (March 4, 2014); doi:10.1117/12.2040985; http://dx.doi.org/10.1117/12.2040985


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