Towards a comprehensive eye model for zebrafish retinal imaging using full range spectral domain optical coherence tomography

Maria Gaertner; Anke Weber; Peter Cimalla; Felix Köttig; Michael Brand; Edmund Koch

doi:10.1117/12.2037588

4 March 2014 Towards a comprehensive eye model for zebrafish retinal imaging using full range spectral domain optical coherence tomography

Maria Gaertner, Anke Weber, Peter Cimalla, Felix Köttig, Michael Brand, Edmund Koch

Author Affiliations +

Proceedings Volume 8934, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XVIII; 89342H (2014) https://doi.org/10.1117/12.2037588
Event: SPIE BiOS, 2014, San Francisco, California, United States

Abstract

In regenerative medicine, the zebrafish is a prominent animal model for studying degeneration and regeneration processes, e.g. of photoreceptor cells in the retina. By means of optical coherence tomography (OCT), these studies can be conducted over weeks using the same individual and hence reducing the variability of the results. To allow an improvement of zebrafish retinal OCT imaging by suitable optics, we developed a zebrafish eye model using geometrical data obtained by in vivo dispersion encoded full range OCT as well as a dispersion comprising gradient index (GRIN) lens model based on refractive index data found in the literature. Using non-sequential ray tracing, the focal length of the spherical GRIN lens (diameter of 0.96 mm) was determined to be 1.22 mm at 800 nm wavelength giving a Matheissen’s ratio (ratio of focal length to radius of the lens) of 2.54, which fits well into the range between 2.19 and 2.82, found for various fish lenses. Additionally, a mean refractive index of 1.64 at 800 nm could be retrieved for the lens to yield the same focal position as found for the GRIN condition. With the aid of the zebrafish eye model, the optics of the OCT scanner head were adjusted to provide high-resolution retinal images with a field of view of 30° x 30°. The introduced model therefore provides the basis for improved retinal imaging with OCT and can be further used to study the image formation within the zebrafish eye.

Citation Download Citation

Maria Gaertner, Anke Weber, Peter Cimalla, Felix Köttig, Michael Brand, and Edmund Koch "Towards a comprehensive eye model for zebrafish retinal imaging using full range spectral domain optical coherence tomography", Proc. SPIE 8934, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XVIII, 89342H (4 March 2014); https://doi.org/10.1117/12.2037588

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