Ptychography: use of quantitative phase information for high-contrast label free time-lapse imaging of living cells

Rakesh Suman; Peter O'Toole

doi:10.1117/12.2037670

4 March 2014 Ptychography: use of quantitative phase information for high-contrast label free time-lapse imaging of living cells

Rakesh Suman, Peter O'Toole

Author Affiliations +

Proceedings Volume 8947, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XII; 89471Z (2014) https://doi.org/10.1117/12.2037670
Event: SPIE BiOS, 2014, San Francisco, California, United States

Abstract

Here we report a novel label free, high contrast and quantitative method for imaging live cells. The technique reconstructs an image from overlapping diffraction patterns using a ptychographical algorithm. The algorithm utilises both amplitude and phase data from the sample to report on quantitative changes related to the refractive index (RI) and thickness of the specimen. We report the ability of this technique to generate high contrast images, to visualise neurite elongation in neuronal cells, and to provide measure of cell proliferation.

Citation Download Citation

Rakesh Suman and Peter O'Toole "Ptychography: use of quantitative phase information for high-contrast label free time-lapse imaging of living cells", Proc. SPIE 8947, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XII, 89471Z (4 March 2014); https://doi.org/10.1117/12.2037670

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