Fluorescent cyanine probe for DNA detection and cellular imaging

Yong-Chao Zheng; Mei-Ling Zheng; Zhen-Sheng Zhao; Xuan-Ming Duan

doi:10.1117/12.2038274

4 March 2014 Fluorescent cyanine probe for DNA detection and cellular imaging

Yong-Chao Zheng, Mei-Ling Zheng, Zhen-Sheng Zhao, Xuan-Ming Duan

Proceedings Volume 8947, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XII; 894722 (2014) https://doi.org/10.1117/12.2038274
Event: SPIE BiOS, 2014, San Francisco, California, United States

Abstract

In our study, two carbazole-based cyanines, 3,6-bis[2-(1-methylpyridinium)vinyl]-9-methyl carbazole diiodide (A) and 6,6'-bis[2-(1-methylpyridinium)vinyl]-bis(9-methyl-carbazol-3yl)methane diiodide (B) were synthesized and employed as light-up probes for DNA and cell imaging. Both of the cyanine probes possess a symmetric structure and bis-cationic center. The obvious induced circular dichroism signals in circular dichroism spectra reveal that the molecules can specifically interact with DNA. Strong fluorescence enhancement is observed when these two cyanines are bound to DNA. These cyanine probes show high binding affinity to oligonucleotides but different binding preferences to various secondary structures. Confocal microscopy images of fixed cell stained by the probes exhibit strong brightness and high contrast in nucleus with a very low cytoplasmic background.

Citation Download Citation

Yong-Chao Zheng, Mei-Ling Zheng, Zhen-Sheng Zhao, and Xuan-Ming Duan "Fluorescent cyanine probe for DNA detection and cellular imaging", Proc. SPIE 8947, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XII, 894722 (4 March 2014); https://doi.org/10.1117/12.2038274

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