Enhancing imaging depth by multi-angle imaging of embryonic structures

Narendran Sudheendran; Chen Wu; Mary E. Dickinson; Irina V. Larina; Kirill V. Larin

doi:10.1117/12.2045529

4 March 2014 Enhancing imaging depth by multi-angle imaging of embryonic structures

Narendran Sudheendran, Chen Wu, Mary E. Dickinson, Irina V. Larina, Kirill V. Larin

Proceedings Volume 8953, Optical Methods in Developmental Biology II; 895306 (2014) https://doi.org/10.1117/12.2045529
Event: SPIE BiOS, 2014, San Francisco, California, United States

Abstract

Because of the ease in generating transgenic/gene knock out models and accessibility to early stages of embryogenesis, mouse and rat models have become invaluable to studying the mechanisms that underlie human birth defects. To study precisely how structural birth defects arise, Ultrasound, MRI, microCT, Optical Projection Tomography (OPT), Optical Coherence Tomography (OCT) and histological methods have all been used for imaging mouse/rat embryos. However, of these methods, only OCT enables live, functional imaging with high spatial and temporal resolution. However, one of the major limitations of conventional OCT imaging is the light depth penetration, which limits acquisition of structural information from the whole embryo. Here we introduce new imaging scheme by OCT imaging from different sides of the embryos that extend the depth penetration of OCT to permit high-resolution imaging of 3D and 4D volumes.

Citation Download Citation

Narendran Sudheendran, Chen Wu, Mary E. Dickinson, Irina V. Larina, and Kirill V. Larin "Enhancing imaging depth by multi-angle imaging of embryonic structures", Proc. SPIE 8953, Optical Methods in Developmental Biology II, 895306 (4 March 2014); https://doi.org/10.1117/12.2045529

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available