Simultaneous real-time quantification of blood flow and vascular growth in the chick embryo using optical coherence tomography

William J. Kowalski; Nikola C. Teslovich; Chia-Yuan Chen; Bradley B. Keller; Kerem Pekkan

doi:10.1117/12.2044932

4 March 2014 Simultaneous real-time quantification of blood flow and vascular growth in the chick embryo using optical coherence tomography

William J. Kowalski, Nikola C. Teslovich, Chia-Yuan Chen, Bradley B. Keller, Kerem Pekkan

Author Affiliations +

Proceedings Volume 8953, Optical Methods in Developmental Biology II; 895307 (2014) https://doi.org/10.1117/12.2044932
Event: SPIE BiOS, 2014, San Francisco, California, United States

Abstract

Experimental and clinical data indicate that hemodynamic forces within the embryo provide critical biomechanical cues for cardiovascular morphogenesis, growth, and remodeling and that perturbed flow is a major etiology of congenital heart disease. However, embryonic flow-growth relationships are largely qualitative and poorly defined. In this work, we provide a quantitative analysis of in vivo flow and growth trends in the chick embryo using optical coherence tomography (OCT) to acquire simultaneous velocity and structural data of the right vitelline artery continuously over a ten hour period beginning at stage 16 (hour 54). We obtained 3D vessel volumes (15 μm lateral, 4.3 μm axial resolutions, 6 μm slice spacing) at 60 minute intervals, taking a B-scan time series totaling one cardiac cycle at each slice. Embryos were maintained at a constant 37°C and 60% humidity during the entire acquisition period through an inhouse built chamber. The 3D vessel lumen geometries were reconstructed manually to assess growth. Blood flow velocity was computed from the central B-scan using red blood cell particle image velocimetry. The use of extended OCT imaging as a non-invasive method for continuous and simultaneous flow and structural data can enhance our understanding of the biomechanical regulation of critical events in morphogenesis. Data acquired will be useful to validate predictive finite-element 3D growth models.

Citation Download Citation

William J. Kowalski, Nikola C. Teslovich, Chia-Yuan Chen, Bradley B. Keller, and Kerem Pekkan "Simultaneous real-time quantification of blood flow and vascular growth in the chick embryo using optical coherence tomography", Proc. SPIE 8953, Optical Methods in Developmental Biology II, 895307 (4 March 2014); https://doi.org/10.1117/12.2044932

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available