Fluorescence-guidance is a useful adjunct to maximize brain tumor resection but current commercial systems are limited by subjective assessment of fluorescence, low sensitivity and non-spectrally-resolved detection. We present a quantitative, spectrally-resolved system integrated onto a commercial neurosurgical microscope that performs spectrallyresolved detection and corrects for effects of tissue optical absorption and scattering on the detected fluorescence signal to image the true fluorophore concentration. Pre-clinical studies in rodent glioma models using multiple fluorophores (PpIX, fluorescein) and clinical studies demonstrate improved residual tumor tissue detection. This quantitative, spectrally-resolved technique opens the door to simultaneous image-guided surgery of multiple fluorophores in the visible and near infrared.
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Pablo A. Valdés ; Valerie L. Jacobs ; Frederic Leblond ; Brian C. Wilson ; Keith D. Paulsen, et al.
Quantitative spectrally resolved intraoperative fluorescence imaging for neurosurgical guidance in brain tumor surgery: pre-clinical and clinical results
", Proc. SPIE 8928, Optical Techniques in Neurosurgery, Neurophotonics, and Optogenetics, 892809 (March 20, 2014); doi:10.1117/12.2039090; http://dx.doi.org/10.1117/12.2039090