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Proceedings Article

Fiber optic fluorescence microscopy for functional brain imaging in awake, mobile mice

[+] Author Affiliations
Jaepyeong Cha, Jin U. Kang

Johns Hopkins Univ. (United States)

Martin Paukert, Dwight E. Bergles

Johns Hopkins School of Medicine (United States)

Proc. SPIE 8928, Optical Techniques in Neurosurgery, Neurophotonics, and Optogenetics, 89282N (March 20, 2014); doi:10.1117/12.2038265
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From Conference Volume 8928

  • Optical Techniques in Neurosurgery, Neurophotonics, and Optogenetics
  • Henry Hirschberg; Steen J. Madsen; E. Duco Jansen; Qingming Luo; Samarendra K. Mohanty; Nitish V. Thakor
  • San Francisco, California, United States | February 01, 2014

abstract

Fiber-optic based optical imaging is an emerging technique for studying brain activity in live animals. Here, we introduce a novel fluorescence fiber-optic microendoscopy approach to minimal invasively detect neural activities in a live mouse brain . The system uses a flexible endoscopic probe composed of a multi-core coherent fiber-bundle terminated with an approximately 1500-micron working distance objective lens. The fiber-optic neural interface is mounted on a 4-mm2 cranial window enabling visualization of glial calcium transients from the same brain region for weeks. We evaluated the system performance through in vivo imaging of GCaMP3 fluorescence in transgenic headrestrained mice during locomotion. © (2014) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Citation

Jaepyeong Cha ; Martin Paukert ; Dwight E. Bergles and Jin U. Kang
" Fiber optic fluorescence microscopy for functional brain imaging in awake, mobile mice ", Proc. SPIE 8928, Optical Techniques in Neurosurgery, Neurophotonics, and Optogenetics, 89282N (March 20, 2014); doi:10.1117/12.2038265; http://dx.doi.org/10.1117/12.2038265


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