Fiber-optic based optical imaging is an emerging technique for studying brain activity in live animals. Here, we
introduce a novel fluorescence fiber-optic microendoscopy approach to minimal invasively detect neural activities in a
live mouse brain . The system uses a flexible endoscopic probe composed of a multi-core coherent fiber-bundle
terminated with an approximately 1500-micron working distance objective lens. The fiber-optic neural interface is
mounted on a 4-mm2 cranial window enabling visualization of glial calcium transients from the same brain region for weeks. We evaluated the system performance through in vivo imaging of GCaMP3 fluorescence in transgenic headrestrained
mice during locomotion.
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Jaepyeong Cha ; Martin Paukert ; Dwight E. Bergles and Jin U. Kang
Fiber optic fluorescence microscopy for functional brain imaging in awake, mobile mice
", Proc. SPIE 8928, Optical Techniques in Neurosurgery, Neurophotonics, and Optogenetics, 89282N (March 20, 2014); doi:10.1117/12.2038265; http://dx.doi.org/10.1117/12.2038265