Method of measuring nitric oxide release by vascular endothelial cells grown in microfluidic channels

S. Hosseinpour; A. C. Liu; A. I. Barakat; J. C. Choy; B. L. Gray

doi:10.1117/12.2038717

6 March 2014 Method of measuring nitric oxide release by vascular endothelial cells grown in microfluidic channels

S. Hosseinpour, A. C. Liu, A. I. Barakat, J. C. Choy, B. L. Gray

Proceedings Volume 8976, Microfluidics, BioMEMS, and Medical Microsystems XII; 89761B (2014) https://doi.org/10.1117/12.2038717
Event: SPIE MOEMS-MEMS, 2014, San Francisco, California, United States

Abstract

In this paper, a simple and versatile method is presented which enables detection of nitric oxide (NO) released from vascular endothelial cells (ECs) cultured in microfluidic structures. The culturing system and NO measurement method allow cell shape to be controlled in a non-invasive manner using microfluidic structures while NO release is monitored for cell shape versus function studies. The culturing system consists of arrays of polydimethylsiloxane (PDMS) fluidic channels 120 micrometers in depth and ranging from 100 micrometers to 3 mm in width. The number of channels in each array is varied to yield a constant cell culture surface area (75 mm²) independent of channel width. The channel surfaces are collagen-coated and ECs are cultured to confluence within the channels. A cell scraper is then used to scrape extraneous cells cultured between channels, and NO measurements are made 18 to 24 hours later. A chemiluminescence-based sensor system (NOA 280i, Sievers NO Analyzer) is utilized to measure sample NO. Initial results indicate that NO concentrations can be measured from different microfluidic channel-containing samples using this method. It is shown that there is no significant difference in NO concentration derived from channels of different widths even though the degree of cell elongation varies due to physical constraint by microfluidic channel walls. However, cells treated with TNFα release more NO than untreated cells in fluidic channels, which is comparable to the function of ECs cultured in conventional culturing systems such as culturing dishes.

Citation Download Citation

S. Hosseinpour, A. C. Liu, A. I. Barakat, J. C. Choy, and B. L. Gray "Method of measuring nitric oxide release by vascular endothelial cells grown in microfluidic channels", Proc. SPIE 8976, Microfluidics, BioMEMS, and Medical Microsystems XII, 89761B (6 March 2014); https://doi.org/10.1117/12.2038717

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
12 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Microfluidics

Silicon

Sensors

NOx

Optical isolators

Photography

Semiconducting wafers

Show All Keywords

Keywords/Phrases

Search In:

Publication Years