Dynamic imaging of transient bubbles generated by femtosecond irradiation of plasmonic nanoparticles in suspensions and cell environment

Christos Boutopoulos; Matthieu Fortin-Deschênes; Eric Bergeron; Michel Meunier

doi:10.1117/12.2039951

7 March 2014 Dynamic imaging of transient bubbles generated by femtosecond irradiation of plasmonic nanoparticles in suspensions and cell environment

Christos Boutopoulos, Matthieu Fortin-Deschênes, Eric Bergeron, Michel Meunier

Author Affiliations +

Proceedings Volume 8972, Frontiers in Ultrafast Optics: Biomedical, Scientific, and Industrial Applications XIV; 897208 (2014) https://doi.org/10.1117/12.2039951
Event: SPIE LASE, 2014, San Francisco, California, United States

Abstract

Femtosecond (fs) laser generation of submicron bubbles around plasmonic nanoparticles (NPs) plays a key role in advanced laser nanosurgery applications such as cell membrane perforation and cell transfection. In this context, we have developed a pump-probe shadowgraphic ultrafast imaging technique capable of tracking transient bubbles generated by fs irradiation (λ =800 nm, τ = 45 fs) of: (a) plasmonic NPs in suspensions and (b) plasmonic NPs attached to cells. The laser fluence was systematically varied from 100 mJ/cm² to 500 mJ/cm2 to study the effect on the bubble dynamics generated around 100 nm gold NPs (Au NPs). The plasmonic bubble (PB) generation threshold as well as the NPs deformation threshold were defined. Dark field (DF) imaging and scanning electron microscopy (SEM) revealed NP clustering when 100 nm bare Au NPs were incubated with a cancer cell culture. NP clustering was correlated with PB generation using a combined pump-probe and DF imaging approach. The clustering effect resulted in a significant 4- times decrease to the PB generation threshold compared to single NPs. The clustering effect was further investigated by evaluating bare and polyethylene glycol (PEG) functionalized NPs in terms of PB generation efficiency.

Citation Download Citation

Christos Boutopoulos, Matthieu Fortin-Deschênes, Eric Bergeron, and Michel Meunier "Dynamic imaging of transient bubbles generated by femtosecond irradiation of plasmonic nanoparticles in suspensions and cell environment", Proc. SPIE 8972, Frontiers in Ultrafast Optics: Biomedical, Scientific, and Industrial Applications XIV, 897208 (7 March 2014); https://doi.org/10.1117/12.2039951

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