High-contrast 3D microscopic imaging of deep layers in a biological medium

Ahmad Faridian; Giancarlo Pedrini; Wolfgang Osten

doi:10.1117/12.2037310

12 March 2014 High-contrast 3D microscopic imaging of deep layers in a biological medium

Ahmad Faridian, Giancarlo Pedrini, Wolfgang Osten

Proceedings Volume 8949, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXI; 89490H (2014) https://doi.org/10.1117/12.2037310
Event: SPIE BiOS, 2014, San Francisco, California, United States

Abstract

Multilayer imaging of biological specimens is a demanding field of research, but scattering is one of the major obstacles in imaging the internal layers of a specimen. Although in many studies the biological object is assumed to be a weak scatterer, this condition is hardly satisfied for sub-millimeter sized organisms. The scattering medium is inhomogeneously distributed inside the specimen. Therefore, the scattering which occurs in the upper layers of a given internal layer of interest is different from the lower layers. That results in a different amount of collectable information for a specific point in the layer from each view. An opposed view dark-field digital holographic microscope (DHM) has been implemented in this work to collect the information concurrently from both views and increase the image quality. Implementing a DHM system gives the possibility to perform digital refocusing process and obtain multilayer images from each side without depth scanning of the object. The results have been presented and discussed here for a Drosophila embryo.

Citation Download Citation

Ahmad Faridian, Giancarlo Pedrini, and Wolfgang Osten "High-contrast 3D microscopic imaging of deep layers in a biological medium", Proc. SPIE 8949, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXI, 89490H (12 March 2014); https://doi.org/10.1117/12.2037310

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KEYWORDS

Digital holography

Image processing

Image enhancement

Scattering

Image fusion

Microscopy

3D image reconstruction

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