Investigation of the SQUBIC phase mask design for depth-invariant widefield microscopy point-spread function engineering

Ana Doblas; Sharon V. King; Nurmohammed Patwary; Genaro Saavedra; Manuel Martínez-Corral; Chrysanthe Preza

doi:10.1117/12.2038610

12 March 2014 Investigation of the SQUBIC phase mask design for depth-invariant widefield microscopy point-spread function engineering

Ana Doblas, Sharon V. King, Nurmohammed Patwary, Genaro Saavedra, Manuel Martínez-Corral, Chrysanthe Preza

Author Affiliations +

Proceedings Volume 8949, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXI; 894914 (2014) https://doi.org/10.1117/12.2038610
Event: SPIE BiOS, 2014, San Francisco, California, United States

Abstract

Point-spread function engineering (PSF), achieved by placing a phase mask at the pupil plane of the imaging lens to encode the wavefront emerging from an imaging system, can be employed to reduce the impact of spherical aberration (SA) in 3D microscopy. In a previous study, the effect of SA on a confocal scanning microscope using a squared cubic phase mask (SQUBIC) was investigated using computer simulations. Here the effect of the SQUBIC design parameter on the insensitivity of the engineered PSF to SA is investigated using a metric based on the second-order moment of the axial variability of the PSF. We show that it is possible to find the optimum SQUBIC for the insensitization to SA.

Citation Download Citation

Ana Doblas, Sharon V. King, Nurmohammed Patwary, Genaro Saavedra, Manuel Martínez-Corral, and Chrysanthe Preza "Investigation of the SQUBIC phase mask design for depth-invariant widefield microscopy point-spread function engineering", Proc. SPIE 8949, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXI, 894914 (12 March 2014); https://doi.org/10.1117/12.2038610

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