The objective of this study is to evaluate the diagnostic potential of stokes shift (SS) spectroscopy (S3) for normal, precancer and cancerous oral lesions in vivo. The SS spectra were recorded in the 250 – 650 nm spectral range by simultaneously scanning both the excitation and emission wavelengths while keeping a fixed wavelength interval Δλ=20 nm between them. Characteristic, highly resolved peaks and significant spectral differences between normal and different pathological oral lesions observed around 300, 355, 395, and 420 nm which are attributed to tryptophan, collagen, and NADH respectively. Using S3 technique one can obtain the key fluorophores in a single scan and hence they can be targeted as a tumor markers in this study. In order to quantify the altered spectral differences between normal and different pathological oral lesions are verified by different ratio parameters.
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Jeyasingh Ebenezar ; Singaravelu Ganesan ; Prakasrao Aruna and Radhakrishnan Muralinaidu
Noninvasive diagnosis of oral cancer by Stokes shift spectroscopy
", Proc. SPIE 8940, Optical Biopsy XII, 89400L (March 17, 2014); doi:10.1117/12.2036798; http://dx.doi.org/10.1117/12.2036798