Tumor margin detection using optical biopsy techniques

Yan Zhou; Cheng-hui Liu; Jiyou Li; Zhongwu Li; Lixin Zhou; Ke Chen; Yang Pu; Yong He; Ke Zhu; Qingbo Li; Robert R. Alfano

doi:10.1117/12.2038723

17 March 2014 Tumor margin detection using optical biopsy techniques

Yan Zhou, Cheng-hui Liu, Jiyou Li, Zhongwu Li, Lixin Zhou, Ke Chen, Yang Pu, Yong He, Ke Zhu, Qingbo Li, Robert R. Alfano

Author Affiliations +

Proceedings Volume 8940, Optical Biopsy XII; 894014 (2014) https://doi.org/10.1117/12.2038723
Event: SPIE BiOS, 2014, San Francisco, California, United States

Abstract

The aim of this study is to use the Resonance Raman (RR) and fluorescence spectroscopic technique for tumor margin detection with high accuracy based on native molecular fingerprints of breast and gastrointestinal (GI) tissues. This tumor margins detection method utilizes advantages of RR spectroscopic technique in situ and in real-time to diagnose tumor changes providing powerful tools for clinical guiding intraoperative margin assessments and postoperative treatments. The tumor margin detection procedures by RR spectroscopy were taken by scanning lesion from center or around tumor region in ex-vivo to find the changes in cancerous tissues with the rim of normal tissues using the native molecular fingerprints. The specimens used to analyze tumor margins include breast and GI carcinoma and normal tissues. The sharp margin of the tumor was found by the changes of RR spectral peaks within 2 mm distance. The result was verified using fluorescence spectra with 300 nm, 320 nm and 340 nm excitation, in a typical specimen of gastric cancerous tissue within a positive margin in comparison with normal gastric tissues. This study demonstrates the potential of RR and fluorescence spectroscopy as new approaches with labeling free to determine the intraoperative margin assessment.

Citation Download Citation

Yan Zhou, Cheng-hui Liu, Jiyou Li, Zhongwu Li, Lixin Zhou, Ke Chen, Yang Pu, Yong He, Ke Zhu, Qingbo Li, and Robert R. Alfano "Tumor margin detection using optical biopsy techniques", Proc. SPIE 8940, Optical Biopsy XII, 894014 (17 March 2014); https://doi.org/10.1117/12.2038723

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available