Investigation on cytoskeleton dynamics for no-adherent cells subjected to point-like stimuli by digital holographic microscopy and holographic optical trapping

Lisa Miccio; Francesco Merola; Pasquale Memmolo; Martina Mugnano; Sabato Fusco; Paolo A. Netti; Pietro Ferraro

doi:10.1117/12.2052811

8 May 2014 Investigation on cytoskeleton dynamics for no-adherent cells subjected to point-like stimuli by digital holographic microscopy and holographic optical trapping

Lisa Miccio, Francesco Merola, Pasquale Memmolo, Martina Mugnano, Sabato Fusco, Paolo A. Netti, Pietro Ferraro

Author Affiliations +

Proceedings Volume 9129, Biophotonics: Photonic Solutions for Better Health Care IV; 91291V (2014) https://doi.org/10.1117/12.2052811
Event: SPIE Photonics Europe, 2014, Brussels, Belgium

Abstract

Guiding, controlling and studying cellular functions are challenging themes in the biomedical field, as they are fundamental prerequisites for new therapeutic strategies from tissue regeneration to controlled drug delivery. In recent years, multidisciplinary studies in nanotechnology offer new tools to investigate important biophysical phenomena in response to the local physical characteristics of the extracellular environment, some examples are the mechanisms of cell adhesion, migration, communication and differentiation. Indeed for reproducing the features of the extracellular matrix in vitro, it is essential to develop active devices that evoke as much as possible the natural cellular environment. Our investigation is in the framework of studying and clarifying the biophysical mechanisms of the interaction between cells and the microenvironment in which they exist. We implement an optical tweezers setup to investigate cell material interaction and we use Digital Holography as non-invasive imaging technique in microscopy. We exploit Holographic Optical Tweezers arrangement in order to trap and manage functionalized micrometric latex beads to induce mechanical deformation in suspended cells. A lot of papers in literature examine the dynamics of the cytoskeleton when cells adhere on substrates and nowadays well established cell models are based on such research activities. Actually, the natural cell environment is made of a complex extracellular matrix and the single cell behavior is due to intricate interactions with the environment and are strongly correlated to the cell-cell interactions. Our investigation is devoted to understand the inner cell mechanism when it is mechanically stressed by point-like stimulus without the substrate influence.

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Lisa Miccio, Francesco Merola, Pasquale Memmolo, Martina Mugnano, Sabato Fusco, Paolo A. Netti, and Pietro Ferraro "Investigation on cytoskeleton dynamics for no-adherent cells subjected to point-like stimuli by digital holographic microscopy and holographic optical trapping", Proc. SPIE 9129, Biophotonics: Photonic Solutions for Better Health Care IV, 91291V (8 May 2014); https://doi.org/10.1117/12.2052811

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KEYWORDS

Digital holography

Holography

Optical tweezers

Cytoskeletons

Microscopy

Optical tracking

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