Direct imaging of singlet oxygen luminescence generated in blood vessels during photodynamic therapy

Lisheng Lin; Huiyun Lin; Defu Chen; Longchao Chen; Min Wang; Shusen Xie; Ying Gu; Brian C. Wilson; Buhong Li

doi:10.1117/12.2052033

8 May 2014 Direct imaging of singlet oxygen luminescence generated in blood vessels during photodynamic therapy

Lisheng Lin, Huiyun Lin, Defu Chen, Longchao Chen, Min Wang, Shusen Xie, Ying Gu, Brian C. Wilson, Buhong Li

Proceedings Volume 9129, Biophotonics: Photonic Solutions for Better Health Care IV; 912920 (2014) https://doi.org/10.1117/12.2052033
Event: SPIE Photonics Europe, 2014, Brussels, Belgium

Abstract

Singlet oxygen (¹O₂) is commonly recognized to be a major phototoxic component for inducing the biological damage during photodynamic therapy (PDT). In this study, a novel configuration of a thermoelectrically-cooled near-infrared sensitive InGaAs camera was developed for imaging of photodynamically-generated ¹O₂ luminescence. The validation of ¹O₂ luminescence images for solution samples was performed with the model photosensitizer Rose Bengal (RB). Images of ¹O₂ luminescence generated in blood vessels in vivo in a well-controlled dorsal skinfold window chamber model were also recorded during PDT. This study demonstrated the capacity of the newly-developed imaging system for imaging of ¹O₂ luminescence, and the first reported images of ¹O₂ luminescence in blood vessels in vivo. This system has potential for elucidating the mechanisms of vascular targeted PDT.

Citation Download Citation

Lisheng Lin, Huiyun Lin, Defu Chen, Longchao Chen, Min Wang, Shusen Xie, Ying Gu, Brian C. Wilson, and Buhong Li "Direct imaging of singlet oxygen luminescence generated in blood vessels during photodynamic therapy", Proc. SPIE 9129, Biophotonics: Photonic Solutions for Better Health Care IV, 912920 (8 May 2014); https://doi.org/10.1117/12.2052033

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