Raman spectroscopic analysis of whole blood acetylcholinesterase

Phillip G. Wilcox; Jin U. Kang

doi:10.1117/12.2052701

5 June 2014 Raman spectroscopic analysis of whole blood acetylcholinesterase

Phillip G. Wilcox, Jin U. Kang

Proceedings Volume 9112, Sensing Technologies for Global Health, Military Medicine, and Environmental Monitoring IV; 91120V (2014) https://doi.org/10.1117/12.2052701
Event: SPIE Sensing Technology + Applications, 2014, Baltimore, MD, United States

Abstract

Raman spectra were taken from whole sheep’s blood with varying levels of acetylcholinesterase (AChE) inhibition using 229 and 532 nm laser excitation wavelengths. AChE levels were inhibited using the organophosphates malathion, paraoxon-ethyl, and octamethyldiphosphoramide and confirmed using the Ellman method. This AChE activity level was investigated with the Raman spectra and analyzed using a partial least squares calibration and cross validation to determine if the AChE activity could be predicted from the Raman spectrum. Correlation scores of 0.78 and 0.26 between the measured and predicted AChE activity were observed using 229 and 532 nm excitation, respectively. A estimate limit of detection was found to be approximately 0.01 ΔA/min.

Citation Download Citation

Phillip G. Wilcox and Jin U. Kang "Raman spectroscopic analysis of whole blood acetylcholinesterase", Proc. SPIE 9112, Sensing Technologies for Global Health, Military Medicine, and Environmental Monitoring IV, 91120V (5 June 2014); https://doi.org/10.1117/12.2052701

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available