ITIA: tripling the field-of-view in off-axis interferometric phase microscopy

Irena Frenklach; Pinhas Girshovitz; Natan T. Shaked

doi:10.1117/12.2064444

18 August 2014 ITIA: tripling the field-of-view in off-axis interferometric phase microscopy

Irena Frenklach, Pinhas Girshovitz, Natan T. Shaked

Proceedings Volume 9203, Interferometry XVII: Techniques and Analysis; 920307 (2014) https://doi.org/10.1117/12.2064444
Event: SPIE Optical Engineering + Applications, 2014, San Diego, California, United States

Abstract

Interferometric phase microscopy (IPM) is a quantitative optical imaging method for capturing the phase profiles of thin samples. While being an invaluable tool for biological and medical research, most IPM setups are unfriendly for inexperienced users, and have limited field of view (FOV). To overcome the limited FOV problem, it is possible to scan the sample and record a wider FOV. However, dynamic samples might move by the time the scan is over. Here, we review our previously published work presenting a new quantitative imaging technique, referred to as interferometry with tripled-imaging area (ITIA), which is capable of capturing three off-axis interferometric fields of view in a single camera exposure, thus tripling the acquired information without the need to scan the sample, without decreasing the image resolution, and without changing the system magnification. Our experimental demonstrations were done by using an inverted transmission microscope illuminated by a Helium-Neon laser. The sample is projected onto the image plane at the output of the microscope, where the ITIA module is placed. Various biological and non-biological samples were imaged.

Citation Download Citation

Irena Frenklach, Pinhas Girshovitz, and Natan T. Shaked "ITIA: tripling the field-of-view in off-axis interferometric phase microscopy", Proc. SPIE 9203, Interferometry XVII: Techniques and Analysis, 920307 (18 August 2014); https://doi.org/10.1117/12.2064444

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KEYWORDS

Interferometry

Beam splitters

Cameras

Einsteinium

Multiplexing

Microscopes

Retroreflectors

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