The whispering gallery mode biosensor: label-free detection from virus to single protein

S. Holler; V. R. Dantham; D. Keng; V. Kolchenko; S. Arnold; Brigid Mulroe; M. Paspaley-Grbavac

doi:10.1117/12.2061477

28 August 2014 The whispering gallery mode biosensor: label-free detection from virus to single protein

S. Holler, V. R. Dantham, D. Keng, V. Kolchenko, S. Arnold, Brigid Mulroe, M. Paspaley-Grbavac

Proceedings Volume 9166, Biosensing and Nanomedicine VII; 91660O (2014) https://doi.org/10.1117/12.2061477
Event: SPIE NanoScience + Engineering, 2014, San Diego, California, United States

Abstract

The whispering gallery mode (WGM) biosensor is a micro-optical platform capable of sensitive label-free detection of biological particles. Described by the reactive sensing principle (RSP), this analytic formulation quantifies the response of the system to the adsorption of bioparticles. Guided by the RSP, the WGM biosensor enabling from detection of virus (e.g., Human Papillomavirus, HPV) to the ultimate goal of single protein detection. The latter was derived from insights into the RSP, which resulted in the development of a hybrid plasmonic WGM biosensor, which has recently demonstrated detection of individual protein cancer markers. Enhancements from bound gold nanoparticles provide the sensitivity to detect single protein molecules (66 kDa) with good signal-to-noise (S/N > 10), and project that detection of proteins as small as 5 kDa.

Citation Download Citation

S. Holler, V. R. Dantham, D. Keng, V. Kolchenko, S. Arnold, Brigid Mulroe, and M. Paspaley-Grbavac "The whispering gallery mode biosensor: label-free detection from virus to single protein", Proc. SPIE 9166, Biosensing and Nanomedicine VII, 91660O (28 August 2014); https://doi.org/10.1117/12.2061477

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