Imaging of surgical margin in pancreatic metastasis using two-photon excited fluorescence microscopy

Jing Chen; Zhipeng Hong; Hong Chen; Youting Chen; Yahao Xu; Xiaoqin Zhu; Shuangmu Zhuo; Zheng Shi; Jianxin Chen

doi:10.1117/12.2068497

17 September 2014 Imaging of surgical margin in pancreatic metastasis using two-photon excited fluorescence microscopy

Jing Chen, Zhipeng Hong, Hong Chen, Youting Chen, Yahao Xu, Xiaoqin Zhu, Shuangmu Zhuo, Zheng Shi, Jianxin Chen

Proceedings Volume 9230, Twelfth International Conference on Photonics and Imaging in Biology and Medicine (PIBM 2014); 92301L (2014) https://doi.org/10.1117/12.2068497
Event: Twelfth International Conference on Photonics and Imaging in Biology and Medicine (PIBM 2014), 2014, Wuhan, China

Abstract

Two-photon excited fluorescence (TPEF) microscopy, has become a powerful tool for imaging unstained tissue samples at subcellular level in biomedical research. The purpose of this study was to determine whether TPEF imaging of histological sections without H-E staining can be used to identify the boundary between normal pancreas and pancreatic metastasis from renal cell carcinoma (RCC). The typical features such as the significant increase of cancerous nests, the absence of pancreatic ductal, the appearance of cancer cells were observed to present the boundary between normal pancreas and pancreatic metastasis from RCC. These results correlated well with the corresponding histological outcomes. With the advent of clinically miniaturized TPEF microscopy and integrative endoscopy, TPEF microscopy has the potential application on surgical location of pancreatic metastasis from RCC in the near future.

Citation Download Citation

Jing Chen, Zhipeng Hong, Hong Chen, Youting Chen, Yahao Xu, Xiaoqin Zhu, Shuangmu Zhuo, Zheng Shi, and Jianxin Chen "Imaging of surgical margin in pancreatic metastasis using two-photon excited fluorescence microscopy", Proc. SPIE 9230, Twelfth International Conference on Photonics and Imaging in Biology and Medicine (PIBM 2014), 92301L (17 September 2014); https://doi.org/10.1117/12.2068497

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KEYWORDS

Microscopy

Tissues

Pancreas

Luminescence

Surgery

Pancreatic cancer

Cancer

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