Signal reduction in fluorescence imaging using radio frequency-multiplexed excitation by compressed sensing

Antony C. S. Chan; Edmund Y. Lam; Kevin K. Tsia

doi:10.1117/12.2072016

13 November 2014 Signal reduction in fluorescence imaging using radio frequency-multiplexed excitation by compressed sensing

Antony C. S. Chan, Edmund Y. Lam, Kevin K. Tsia

Author Affiliations +

Proceedings Volume 9279, Real-time Photonic Measurements, Data Management, and Processing; 92790U (2014) https://doi.org/10.1117/12.2072016
Event: SPIE/COS Photonics Asia, 2014, Beijing, China

Abstract

Fluorescence imaging using radio frequency-multiplexed excitation (FIRE) has emerged to enable an order-of-magnitude higher frame rate than the current technologies. Similar to all high-speed realtime imaging modalities, FIRE inherently generates massive image data continuously. While this technology entails high-throughput data sampling, processing, and storage in real-time, strategies in data compression on the fly is also beneficial. We here report that it is feasible to exploit the radio frequency-multiplexed excitation scheme in FIRE for implementing compressed sensing (CS) without any modification of the FIRE system. We numerically demonstrate that CS-FIRE can reduce the effective data rate by 95% without severe degradation of image quality.

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Antony C. S. Chan, Edmund Y. Lam, and Kevin K. Tsia "Signal reduction in fluorescence imaging using radio frequency-multiplexed excitation by compressed sensing", Proc. SPIE 9279, Real-time Photonic Measurements, Data Management, and Processing, 92790U (13 November 2014); https://doi.org/10.1117/12.2072016

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