Measurement of refractive index distribution of biotissues by scanning focused refractive index microscopy

Tengqian Sun; Qing Ye; Xiao-Wan Wang; Jin Wang; Zhi-Chao Deng; Jian-Chun Mei; Wen-Yuan Zhou; Chun-Ping Zhang; Jian-Guo Tian

doi:10.1117/12.2072026

18 November 2014 Measurement of refractive index distribution of biotissues by scanning focused refractive index microscopy

Tengqian Sun, Qing Ye, Xiao-Wan Wang, Jin Wang, Zhi-Chao Deng, Jian-Chun Mei, Wen-Yuan Zhou, Chun-Ping Zhang, Jian-Guo Tian

Author Affiliations +

Proceedings Volume 9268, Optics in Health Care and Biomedical Optics VI; 926813 (2014) https://doi.org/10.1117/12.2072026
Event: SPIE/COS Photonics Asia, 2014, Beijing, China

Abstract

We adapt the improved scanning focused refractive-index microscopy (SFRIM) technique to the quantitative study of biological tissues. Delicate refractive index (RI) imaging of a porcine muscle tissue is obtained in a reflection mode. Some modifications are made to the SFRIM for better two dimension (2-D) observation of the tissues. The RI accuracy is 0.002. The central spatial resolution of SFRIM achieves 1μm, smaller than the size of the focal spot. Our method is free from signal distortion. The experimental result demonstrates that SFRIM is a potential technique in a wide field of biomedical research.

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Tengqian Sun, Qing Ye, Xiao-Wan Wang, Jin Wang, Zhi-Chao Deng, Jian-Chun Mei, Wen-Yuan Zhou, Chun-Ping Zhang, and Jian-Guo Tian "Measurement of refractive index distribution of biotissues by scanning focused refractive index microscopy", Proc. SPIE 9268, Optics in Health Care and Biomedical Optics VI, 926813 (18 November 2014); https://doi.org/10.1117/12.2072026

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KEYWORDS

Tissues

Refractive index

Prisms

Microscopy

Sensors

Spatial resolution

Charge-coupled devices

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