WP1: transgenic opto-animals

E. Użarowska; Rafał Czajkowski; W. Konopka

doi:10.1117/12.2075210

25 November 2014 WP1: transgenic opto-animals

E. Użarowska, Rafał Czajkowski, W. Konopka

Proceedings Volume 9290, Photonics Applications in Astronomy, Communications, Industry, and High-Energy Physics Experiments 2014; 92901V (2014) https://doi.org/10.1117/12.2075210
Event: Symposium on Photonics Applications in Astronomy, Communications, Industry and High-Energy Physics Experiments, 2014, Warsaw, Poland

Abstract

We aim to create a set of genetic tools where permanent opsin expression (ChR or NpHR) is precisely limited to the population of neurons that express immediate early gene c-fos during a specific temporal window of behavioral training. Since the c-fos gene is only expressed in neurons that form experience-dependent ensemble, this approach will result in specific labeling of a small subset of cells that create memory trace for the learned behavior. To this end we employ two alternative inducible gene expression systems: Tet Expression System and Cre/lox System. In both cases, the temporal window for opsin induction is controlled pharmacologically, by doxycycline or tamoxifen, respectively. Both systems will be used for creating lines of transgenic animals.

Citation Download Citation

E. Użarowska, Rafał Czajkowski, and W. Konopka "WP1: transgenic opto-animals", Proc. SPIE 9290, Photonics Applications in Astronomy, Communications, Industry, and High-Energy Physics Experiments 2014, 92901V (25 November 2014); https://doi.org/10.1117/12.2075210

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KEYWORDS

Neurons

Proteins

Brain

Optogenetics

Genetics

Chemical elements

Control systems

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