Reproducible and time-course study of  yeast biofilm by Raman spectroscopy

Ota Samek; Katarina Mlynariková; Silvie Bernatová; Jan Ježek; Vladislav Kryžánek; Martin Šiler; Pavel Zemánek; Filip Růžička

doi:10.1117/12.2176031

5 December 2014 Reproducible and time-course study of yeast biofilm by Raman spectroscopy

Ota Samek, Katarina Mlynariková, Silvie Bernatová, Jan Ježek, Vladislav Kryžánek, Martin Šiler, Pavel Zemánek, Filip Růžička

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Proceedings Volume 9441, 19th Polish-Slovak-Czech Optical Conference on Wave and Quantum Aspects of Contemporary Optics; 94411M (2014) https://doi.org/10.1117/12.2176031
Event: XIX Polish-Slovak-Czech Optical Conference on Wave and Quantum Aspects of Contemporary Optics, 2014, Jelenia Gora, Poland

Abstract

We report on Raman spectroscopy measurements - separated by a given time intervals - for the selected yeast strains (biofilm positive and biofilm negative) on colonies grown directly on the Petri dishes or on the well-plate. Chemometric principal component analysis of these spectra sets generated clusters of data points, from which the reproducibility of the measurement could be analysed. Consequently, these resulted in clusters coinciding well with the biofilm positive and biofilm negative strains measurement of a particular sample dish, suggesting good reproducibility of our measurement procedure, even when the samples were prepared and measured days up to months apart. This suggests the potential of Raman spectroscopy in routine clinical diagnostic.

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Ota Samek, Katarina Mlynariková, Silvie Bernatová, Jan Ježek, Vladislav Kryžánek, Martin Šiler, Pavel Zemánek, and Filip Růžička "Reproducible and time-course study of yeast biofilm by Raman spectroscopy", Proc. SPIE 9441, 19th Polish-Slovak-Czech Optical Conference on Wave and Quantum Aspects of Contemporary Optics, 94411M (5 December 2014); https://doi.org/10.1117/12.2176031

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KEYWORDS

Raman spectroscopy

Yeast

Antimicrobial agents

Microfluidics

Principal component analysis

Proteins

Biological research

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