Laser induced autofluorescence for diagnosis of non-melanoma skin cancer

E. Drakaki; M. Makropoulou; A. A. Serafetinides; N. Merlemis; I. Kalatzis; I. A. Sianoudis; O. Batsi; E. Christofidou; A. J. Stratigos; A. D. Katsambas; Ch. Antoniou

doi:10.1117/12.2175647

8 January 2015 Laser induced autofluorescence for diagnosis of non-melanoma skin cancer

E. Drakaki, M. Makropoulou, A. A. Serafetinides, N. Merlemis, I. Kalatzis, I. A. Sianoudis, O. Batsi, E. Christofidou, A. J. Stratigos, A. D. Katsambas, Ch. Antoniou

Author Affiliations +

Proceedings Volume 9447, 18th International School on Quantum Electronics: Laser Physics and Applications; 94470Y (2015) https://doi.org/10.1117/12.2175647
Event: Eighteenth International School on Quantum Electronics: Laser Physics and Applications, 2014, Sozopol, Bulgaria

Abstract

Non melanoma skin cancer is one of the most frequent malignant tumors among humans. A non-invasive technique, with high sensitivity and high specificity, would be the most suitable method for basal cell carcinoma (BCC) or other malignancies diagnostics, instead of the well established biopsy and histopathology examination. In the last decades, a non-invasive, spectroscopic diagnostic method was introduced, the laser induced fluorescence (LIF), which could generate an image contrast between different states of skin tissue. The noninvasiveness consists in that this biophotonic method do not require tissue sample excision, what is necessary in histopathology characterization and biochemical analysis of the skin tissue samples, which is worldwide used as an evaluation gold standard. The object of this study is to establish the possibilities of a relatively portable system for laser induced skin autofluorescence to differentiate malignant from nonmalignant skin lesions. Unstained human skin samples, excised from humans undergoing biopsy examination, were irradiated with a Nd:YAG-3ω laser (λ=355 nm, 6 ns), used as an excitation source for the autofluorescence measurements. A portable fiber-based spectrometer was used to record fluorescence spectra of the sites of interest. The ex vivo results, obtained with this spectroscopic technique, were correlated with the histopathology results. After the analysis of the fluorescence spectra of almost 60 skin tissue areas, we developed an algorithm to distinguish different types of malignant lesions, including inflammatory areas. Optimization of the data analysis and potential use of LIF spectroscopy with 355 nm Nd:YAG laser excitation of tissue autofluorescence for clinical applications are discussed.

Citation Download Citation

E. Drakaki, M. Makropoulou, A. A. Serafetinides, N. Merlemis, I. Kalatzis, I. A. Sianoudis, O. Batsi, E. Christofidou, A. J. Stratigos, A. D. Katsambas, and Ch. Antoniou "Laser induced autofluorescence for diagnosis of non-melanoma skin cancer", Proc. SPIE 9447, 18th International School on Quantum Electronics: Laser Physics and Applications, 94470Y (8 January 2015); https://doi.org/10.1117/12.2175647

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
8 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Skin

Tissues

Luminescence

Spectroscopy

Diagnostics

Laser induced fluorescence

Skin cancer

Show All Keywords

Keywords/Phrases

Search In:

Publication Years