In vivo laser-based imaging of the human fallopian tube for future cancer detection

Eric J. Seibel; Charles D. Melville; Richard S. Johnston; Yuanzheng Gong; Kathy Agnew; Seine Chiang; Elizabeth M. Swisher

doi:10.1117/12.2076972

2 March 2015 In vivo laser-based imaging of the human fallopian tube for future cancer detection

Eric J. Seibel, Charles D. Melville, Richard S. Johnston, Yuanzheng Gong, Kathy Agnew, Seine Chiang, Elizabeth M. Swisher

Author Affiliations +

Proceedings Volume 9304, Endoscopic Microscopy X; and Optical Techniques in Pulmonary Medicine II; 93040Q (2015) https://doi.org/10.1117/12.2076972
Event: SPIE BiOS, 2015, San Francisco, California, United States

Abstract

Inherited mutations in BRCA1 and BRCA2 lead to 20-50% lifetime risk of ovarian, tubal, or peritoneal carcinoma. Clinical recommendations for women with these genetic mutations include the prophylactic removal of ovaries and fallopian tubes by age 40 after child-bearing. Recent findings suggest that many presumed ovarian or peritoneal carcinomas arise in fallopian tube epithelium. Although survival rate is <90% when ovarian cancer is detected early (Stage_I), 70% of women have advanced disease (Stage_III/IV) at presentation when survival is less than 30%. Over the years, effective early detection of ovarian cancer has remained elusive, possibly because screening techniques have mistakenly focused on the ovary as origin of ovarian carcinoma. Unlike ovaries, the fallopian tubes are amenable to direct visual imaging without invasive surgery, using access through the cervix. To develop future screening protocols, we investigated using our 1.2- mm diameter, forward-viewing, scanning fiber endoscope (SFE) to image luminal surfaces of the fallopian tube before laparoscopic surgical removal. Three anesthetized human subjects participated in our protocol development which eventually led to 70-80% of the length of fallopian tubes being imaged in scanning reflectance, using red (632nm), green (532nm), and blue (442nm) laser light. A hysteroscope with saline uterine distention was used to locate the tubal ostia. To facilitate passage of the SFE through the interstitial portion of the fallopian tube, an introducer catheter was inserted 1- cm through each ostia. During insertion, saline was flushed to reduce friction and provide clearer viewing. This is likely the first high-resolution intraluminal visualization of fallopian tubes.

Citation Download Citation

Eric J. Seibel, Charles D. Melville, Richard S. Johnston, Yuanzheng Gong, Kathy Agnew, Seine Chiang, and Elizabeth M. Swisher "In vivo laser-based imaging of the human fallopian tube for future cancer detection", Proc. SPIE 9304, Endoscopic Microscopy X; and Optical Techniques in Pulmonary Medicine II, 93040Q (2 March 2015); https://doi.org/10.1117/12.2076972

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available