A six-color four-laser mobile platform for multi-spectral fluorescence imaging endoscopy

John F. Black; Tyler Tate; Molly Keenan; Elizabeth Swan; Urs Utzinger; Jennifer Barton

doi:10.1117/12.2077999

2 March 2015 A six-color four-laser mobile platform for multi-spectral fluorescence imaging endoscopy

John F. Black, Tyler Tate, Molly Keenan, Elizabeth Swan, Urs Utzinger, Jennifer Barton

Proceedings Volume 9304, Endoscopic Microscopy X; and Optical Techniques in Pulmonary Medicine II; 93040T (2015) https://doi.org/10.1117/12.2077999
Event: SPIE BiOS, 2015, San Francisco, California, United States

Abstract

The properties of multi-spectral fluorescence imaging using deep-UV-illumination have recently been explored using a fiber-coupled thermal source at 280 nm. The resulting images show a remarkable level of contrast thought to result from the signal being overwhelmingly generated in the uppermost few cell layers of tissue, making this approach valuable for the study of diseases that originate in the endothelial tissues of the body. With a view to extending the technique with new wavelengths, and improving beam quality for efficient small core fiber coupling we have developed a mobile self-contained tunable solid-state laser source of deep UV light. An alexandrite laser, lasing at around 750 nm is frequency doubled to produce 375 nm and then tripled to produce 250 nm light. An optical deck added to the system allows other laser sources to be incorporated into the UV beam-line and a lens system has been designed to couple these sources into a single delivery fiber with core diameters down to 50 microns. Our system incorporates five wavelengths [250 nm, 375 nm, 442 nm (HeCd), 543 nm (HeNe) and 638 nm (diode laser)] as the illumination source for a small diameter falloposcope designed for the study of the distal Fallopian tube origins of high grade serous ovarian cancer. The tunability of alexandrite offers the potential to generate other wavelengths in the 720–800, 360–400 and 240–265 nm ranges, plus other non-linear optical conversion techniques taking advantage of the high peak powers of the laser.

Citation Download Citation

John F. Black, Tyler Tate, Molly Keenan, Elizabeth Swan, Urs Utzinger, and Jennifer Barton "A six-color four-laser mobile platform for multi-spectral fluorescence imaging endoscopy", Proc. SPIE 9304, Endoscopic Microscopy X; and Optical Techniques in Pulmonary Medicine II, 93040T (2 March 2015); https://doi.org/10.1117/12.2077999

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