Label-free analysis of single and multiple cells with a 2D light scattering static cytometer

Shanshan Liu; Linyan Xie; Yan Yang; Xu Qiao; Kun Song; Beihua Kong; Xuantao Su

doi:10.1117/12.2080519

2 March 2015 Label-free analysis of single and multiple cells with a 2D light scattering static cytometer

Shanshan Liu, Linyan Xie, Yan Yang, Xu Qiao, Kun Song, Beihua Kong, Xuantao Su

Proceedings Volume 9328, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XIII; 93281B (2015) https://doi.org/10.1117/12.2080519
Event: SPIE BiOS, 2015, San Francisco, California, United States

Abstract

Cytometry has wide applications in biomedicine for cell differentiation or disease monitoring. Here we report our newly developed two dimensional (2D) light scattering static cytometric technique for single and multiple cell analysis. The static cytometer adopts a scanning fiber probe for cell excitation and obtains 2D light scattering patterns on a complementary metal oxide semiconductor (CMOS) detector. Our results show that experimental 2D light scattering patterns obtained from single yeast cells are with fringe structure while those from multiple yeast cells give speckle patterns. The experimental results compare favorably with our 2D light scattering Mie theory simulations for both single and multiple cells. The varying of 2D light scattering patterns with different yeast cell clusters, either number or distribution changes, shows the potential of our 2D light scattering static cytometer for cellular diagnostics.

Citation Download Citation

Shanshan Liu, Linyan Xie, Yan Yang, Xu Qiao, Kun Song, Beihua Kong, and Xuantao Su "Label-free analysis of single and multiple cells with a 2D light scattering static cytometer", Proc. SPIE 9328, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XIII, 93281B (2 March 2015); https://doi.org/10.1117/12.2080519

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KEYWORDS

Light scattering

Yeast

Mie scattering

Speckle pattern

Biological research

CMOS sensors

Diagnostics

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