Deep stroma investigation by confocal microscopy

Francesca Rossi; Francesca Tatini; Roberto Pini; Paola Valente; Roberta Ardia; Luca Buzzonetti; Annalisa Canovetti; Alex Malandrini; Ivo Lenzetti; Luca Menabuoni M.D.

doi:10.1117/12.2077112

20 March 2015 Deep stroma investigation by confocal microscopy

Francesca Rossi, Francesca Tatini, Roberto Pini, Paola Valente, Roberta Ardia, Luca Buzzonetti, Annalisa Canovetti, Alex Malandrini, Ivo Lenzetti, Luca Menabuoni M.D.

Author Affiliations +

Proceedings Volume 9307, Ophthalmic Technologies XXV; 93071H (2015) https://doi.org/10.1117/12.2077112
Event: SPIE BiOS, 2015, San Francisco, California, United States

Abstract

Laser assisted keratoplasty is nowadays largely used to perform minimally invasive surgery and partial thickness keratoplasty [1-3]. The use of the femtosecond laser enables to perform a customized surgery, solving the specific problem of the single patient, designing new graft profiles and partial thickness keratoplasty (PTK). The common characteristics of the PTKs and that make them eligible respect to the standard penetrating keratoplasty, are: the preservation of eyeball integrity, a reduced risk of graft rejection, a controlled postoperative astigmatism. On the other hand, the optimal surgical results after these PTKs are related to a correct comprehension of the deep stroma layers morphology, which can help in the identification of the correct cleavage plane during surgeries. In the last years some studies were published, giving new insights about the posterior stroma morphology in adult subjects [4,5]. In this work we present a study performed on two groups of tissues: one group is from 20 adult subjects aged 59 ± 18 y.o., and the other group is from 15 young subjects, aged 12±5 y.o.. The samples were from tissues not suitable for transplant in patients. Confocal microscopy and Environmental Scanning Electron Microscopy (ESEM) were used for the analysis of the deep stroma. The preliminary results of this analysis show the main differences in between young and adult tissues, enabling to improve the knowledge of the morphology and of the biomechanical properties of human cornea, in order to improve the surgical results in partial thickness keratoplasty.

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Francesca Rossi, Francesca Tatini, Roberto Pini, Paola Valente, Roberta Ardia, Luca Buzzonetti, Annalisa Canovetti, Alex Malandrini, Ivo Lenzetti, and Luca Menabuoni M.D. "Deep stroma investigation by confocal microscopy", Proc. SPIE 9307, Ophthalmic Technologies XXV, 93071H (20 March 2015); https://doi.org/10.1117/12.2077112

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