Broadband characterization of tissue simulating phantoms using a supercontinuum laser in a scanning diffuse optical spectroscopy instrument

Albert E. Cerussi; Kevin Conde; Jesse Lam; Vaibhav Verma

doi:10.1117/12.2078436

5 March 2015 Broadband characterization of tissue simulating phantoms using a supercontinuum laser in a scanning diffuse optical spectroscopy instrument

Albert E. Cerussi, Kevin Conde, Jesse Lam, Vaibhav Verma

Author Affiliations +

Proceedings Volume 9319, Optical Tomography and Spectroscopy of Tissue XI; 93191Z (2015) https://doi.org/10.1117/12.2078436
Event: SPIE BiOS, 2015, San Francisco, California, United States

Abstract

Near Infrared (NIR) optical spectroscopy and imaging technologies are increasingly applied to clinical and pre-clinical applications due to their strong metabolic sensitivity. These technologies rely upon calibrations to remove system artifacts. The most common calibration method requires a tissue-simulating phantom with known absorption (μ_a) and reduced scattering (μ_s’) coefficients to remove temporal and/or spatial artifacts from the measured data. While this method can be effective under certain conditions, there is no universal agreement on how to measure calibration phantom optical properties. An independent method for measuring calibration phantom broadband optical spectra is desired, especially without calibration with reference to known optical properties. We developed a broadband instrument that recovers absolute μ_a and μ_s’ spectra from 620 to 1050 nm in tissue-simulating phantoms. The instrument scans a supercontinuum laser beam across the phantom surface and collects diffuse reflectance via a spectrometercoupled optical fiber. A Monte Carlo algorithm was used to fit the reflectance as a function of source-detector separation and recover absorption and scattering spectra. The only calibration required is a measurement of the laser spot positions on the phantom surface; no optical properties need be known. Spectral constraints were used to create a robust fitting procedure. We demonstrate experimental repeatability by measuring the same phantom multiple times and recover absolute absorption and scattering spectra with ~10% precision across the entire NIR spectral region.

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Albert E. Cerussi, Kevin Conde, Jesse Lam, and Vaibhav Verma "Broadband characterization of tissue simulating phantoms using a supercontinuum laser in a scanning diffuse optical spectroscopy instrument", Proc. SPIE 9319, Optical Tomography and Spectroscopy of Tissue XI, 93191Z (5 March 2015); https://doi.org/10.1117/12.2078436

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KEYWORDS

Calibration

Optical properties

Monte Carlo methods

Scattering

Tissue optics

Near infrared

Tissues

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