3D-localization microscopy and tracking of FoF1-ATP synthases in living bacteria

Anja Renz; Marc Renz; Diana Klütsch; Gabriele Deckers-Hebestreit; Michael Börsch

doi:10.1117/12.2080981

9 March 2015 3D-localization microscopy and tracking of FoF1-ATP synthases in living bacteria

Anja Renz, Marc Renz, Diana Klütsch, Gabriele Deckers-Hebestreit, Michael Börsch

Proceedings Volume 9331, Single Molecule Spectroscopy and Superresolution Imaging VIII; 93310D (2015) https://doi.org/10.1117/12.2080981
Event: SPIE BiOS, 2015, San Francisco, California, United States

Abstract

F_oF₁-ATP synthases are membrane-embedded protein machines that catalyze the synthesis of adenosine triphosphate. Using photoactivation-based localization microscopy (PALM) in TIR-illumination as well as structured illumination microscopy (SIM), we explore the spatial distribution and track single F_oF₁-ATP synthases in living E. coli cells under physiological conditions at different temperatures. For quantitative diffusion analysis by mean-squared-displacement measurements, the limited size of the observation area in the membrane with its significant membrane curvature has to be considered. Therefore, we applied a 'sliding observation window' approach (M. Renz et al., Proc. SPIE 8225, 2012) and obtained the one-dimensional diffusion coefficient of F_oF₁-ATP synthase diffusing on the long axis in living E. coli cells.

Citation Download Citation

Anja Renz, Marc Renz, Diana Klütsch, Gabriele Deckers-Hebestreit, and Michael Börsch "3D-localization microscopy and tracking of FoF1-ATP synthases in living bacteria", Proc. SPIE 9331, Single Molecule Spectroscopy and Superresolution Imaging VIII, 93310D (9 March 2015); https://doi.org/10.1117/12.2080981

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