Experimental systems for optogenetic control of protein activity with photodissociable fluorescent proteins

Xin X. Zhou; Michael Z. Lin

doi:10.1117/12.2081242

10 March 2015 Experimental systems for optogenetic control of protein activity with photodissociable fluorescent proteins

Xin X. Zhou, Michael Z. Lin

Author Affiliations +

Proceedings Volume 9305, Optical Techniques in Neurosurgery, Neurophotonics, and Optogenetics II; 930534 (2015) https://doi.org/10.1117/12.2081242
Event: SPIE BiOS, 2015, San Francisco, California, United States

Abstract

Fluorescent Light-inducible Proteins are proteins of interest that have Dronpa domains fused to both termini. In the dark, the Dronpa domains tetramerize and cage the protein, but light induces Dronpa dissociation and activates the protein. Here, we describe four experimental systems for light activation of mammalian cells expressing FLiPs.

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Xin X. Zhou and Michael Z. Lin "Experimental systems for optogenetic control of protein activity with photodissociable fluorescent proteins", Proc. SPIE 9305, Optical Techniques in Neurosurgery, Neurophotonics, and Optogenetics II, 930534 (10 March 2015); https://doi.org/10.1117/12.2081242

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