An imaging and analysis toolset for the study of Caenorhabditis elegans neurodevelopment

Ryan Christensen; Alexandra Bokinsky; Anthony Santella; Yicong Wu; Javier Marquina; Ismar Kovacevic; Abhishek Kumar; Peter Winter; Evan McCreedy; William Mohler; Zhirong Bao; Daniel Colón-Ramos; Hari Shroff

doi:10.1117/12.2082394

10 March 2015 An imaging and analysis toolset for the study of Caenorhabditis elegans neurodevelopment

Ryan Christensen, Alexandra Bokinsky, Anthony Santella, Yicong Wu, Javier Marquina, Ismar Kovacevic, Abhishek Kumar, Peter Winter, Evan McCreedy, William Mohler, Zhirong Bao, Daniel Colón-Ramos, Hari Shroff

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Proceedings Volume 9334, Optical Methods in Developmental Biology III; 93340C (2015) https://doi.org/10.1117/12.2082394
Event: SPIE BiOS, 2015, San Francisco, California, United States

Abstract

How an entire nervous system develops remains mysterious. We have developed a light-sheet microscope system to examine neurodevelopment in C. elegans embryos. Our system creates overlapping light sheets from two orthogonally positioned objectives, enabling imaging from the first cell division to hatching (~14 hours) with 350 nm isotropic resolution. We have also developed computer algorithms to computationally straighten nematode embryos, facilitating data comparison and combination from multiple animals. We plan to use these tools to create an atlas showing the position and morphology of all neurons in the developing embryo.

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Ryan Christensen, Alexandra Bokinsky, Anthony Santella, Yicong Wu, Javier Marquina, Ismar Kovacevic, Abhishek Kumar, Peter Winter, Evan McCreedy, William Mohler, Zhirong Bao, Daniel Colón-Ramos, and Hari Shroff "An imaging and analysis toolset for the study of Caenorhabditis elegans neurodevelopment", Proc. SPIE 9334, Optical Methods in Developmental Biology III, 93340C (10 March 2015); https://doi.org/10.1117/12.2082394

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