Comparison of optical projection tomography and optical coherence tomography for assessment of murine embryonic development

Manmohan Singh; Achuth Nair; Tegy Vadakkan; Victor Piazza; Ryan Udan; Michael V. Frazier; Trevor Janecek; Mary E. Dickinson; Kirill V. Larin

doi:10.1117/12.2078319

10 March 2015 Comparison of optical projection tomography and optical coherence tomography for assessment of murine embryonic development

Manmohan Singh, Achuth Nair, Tegy Vadakkan, Victor Piazza, Ryan Udan, Michael V. Frazier, Trevor Janecek, Mary E. Dickinson, Kirill V. Larin

Author Affiliations +

Proceedings Volume 9334, Optical Methods in Developmental Biology III; 93340J (2015) https://doi.org/10.1117/12.2078319
Event: SPIE BiOS, 2015, San Francisco, California, United States

Abstract

The murine model is a common model for studying developmental diseases. In this study, we compare the performance of the relatively new method of Optical Projection Tomography (OPT) to the well-established technique of Optical Coherence Tomography (OCT) to assess murine embryonic development at three stages, 9.5, 11.5, and 13.5 days post conception. While both methods can provide spatial resolution at the micrometer scale, OPT can provide superior imaging depth compared to OCT. However, OPT requires samples to be fixed, placed in an immobilization media such as agar, and cleared before imaging. Because OCT does not require fixing, it can be used to image embryos in vivo and in utero. In this study, we compare the efficacy of OPT and OCT for imaging murine embryonic development. The data demonstrate the superior capability of OPT for imaging fine structures with high resolution in optically-cleared embryos while only OCT can provide structural and functional imaging of live embryos ex vivo and in utero with micrometer scale resolution.

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Manmohan Singh, Achuth Nair, Tegy Vadakkan, Victor Piazza, Ryan Udan, Michael V. Frazier, Trevor Janecek, Mary E. Dickinson, and Kirill V. Larin "Comparison of optical projection tomography and optical coherence tomography for assessment of murine embryonic development", Proc. SPIE 9334, Optical Methods in Developmental Biology III, 93340J (10 March 2015); https://doi.org/10.1117/12.2078319

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