Using digital inline holographic microscopy and quantitative phase contrast imaging to assess viability of cultured mammalian cells

Sergey Missan; Olga Hrytsenko

doi:10.1117/12.2078284

11 March 2015 Using digital inline holographic microscopy and quantitative phase contrast imaging to assess viability of cultured mammalian cells

Sergey Missan, Olga Hrytsenko

Author Affiliations +

Proceedings Volume 9336, Quantitative Phase Imaging; 93361X (2015) https://doi.org/10.1117/12.2078284
Event: SPIE BiOS, 2015, San Francisco, California, United States

Abstract

Digital inline holographic microscopy was used to record holograms of mammalian cells (HEK293, B16, and E0771) in culture. The holograms have been reconstructed using Octopus software (4Deep inwater imaging) and phase shift maps were unwrapped using the FFT-based phase unwrapping algorithm. The unwrapped phase shifts were used to determine the maximum phase shifts in individual cells. Addition of 0.5 mM H₂O₂ to cell media produced rapid rounding of cultured cells, followed by cell membrane rupture. The cell morphology changes and cell membrane ruptures were detected in real time and were apparent in the unwrapped phase shift images. The results indicate that quantitative phase contrast imaging produced by the digital inline holographic microscope can be used for the label-free real time automated determination of cell viability and confluence in mammalian cell cultures.

Citation Download Citation

Sergey Missan and Olga Hrytsenko "Using digital inline holographic microscopy and quantitative phase contrast imaging to assess viability of cultured mammalian cells", Proc. SPIE 9336, Quantitative Phase Imaging, 93361X (11 March 2015); https://doi.org/10.1117/12.2078284

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