Full Content is available to subscribers

Subscribe/Learn More  >
Proceedings Article

High throughput imaging of blood smears using white light diffraction phase microscopy

[+] Author Affiliations
Hassaan Majeed, Mikhail E. Kandel, Basanta Bhaduri, Kevin Han, Zelun Luo, Krishnarao Tangella, Gabriel Popescu

Univ. of Illinois at Urbana-Champaign (United States)

Proc. SPIE 9336, Quantitative Phase Imaging, 93361Y (March 11, 2015); doi:10.1117/12.2080200
Text Size: A A A
From Conference Volume 9336

  • Quantitative Phase Imaging
  • Gabriel Popescu; YongKeun Park
  • San Francisco, California, United States | February 07, 2015

abstract

While automated blood cell counters have made great progress in detecting abnormalities in blood, the lack of specificity for a particular disease, limited information on single cell morphology and intrinsic uncertainly due to high throughput in these instruments often necessitates detailed inspection in the form of a peripheral blood smear. Such tests are relatively time consuming and frequently rely on medical professionals tally counting specific cell types. These assays rely on the contrast generated by chemical stains, with the signal intensity strongly related to staining and preparation techniques, frustrating machine learning algorithms that require consistent quantities to denote the features in question. Instead we opt to use quantitative phase imaging, understanding that the resulting image is entirely due to the structure (intrinsic contrast) rather than the complex interplay of stain and sample. We present here our first steps to automate peripheral blood smear scanning, in particular a method to generate the quantitative phase image of an entire blood smear at high throughput using white light diffraction phase microscopy (wDPM), a single shot and common path interferometric imaging technique. © (2015) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Citation

Hassaan Majeed ; Mikhail E. Kandel ; Basanta Bhaduri ; Kevin Han ; Zelun Luo, et al.
" High throughput imaging of blood smears using white light diffraction phase microscopy ", Proc. SPIE 9336, Quantitative Phase Imaging, 93361Y (March 11, 2015); doi:10.1117/12.2080200; http://dx.doi.org/10.1117/12.2080200


Access This Proceeding
Sign in or Create a personal account to Buy this proceeding ($15 for members, $18 for non-members).

Figures

Tables

NOTE:
Citing articles are presented as examples only. In non-demo SCM6 implementation, integration with CrossRef’s "Cited By" API will populate this tab (http://www.crossref.org/citedby.html).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Related Book Chapters

Topic Collections

Advertisement


 

  • Don't have an account?
  • Subscribe to the SPIE Digital Library
  • Create a FREE account to sign up for Digital Library content alerts and gain access to institutional subscriptions remotely.
Access This Proceeding
Sign in or Create a personal account to Buy this proceeding ($15 for members, $18 for non-members).
Access This Proceeding
Sign in or Create a personal account to Buy this article ($15 for members, $18 for non-members).
Access This Chapter

Access to SPIE eBooks is limited to subscribing institutions and is not available as part of a personal subscription. Print or electronic versions of individual SPIE books may be purchased via SPIE.org.