Biological super-resolution imaging by using novel microsphere-embedded coverslips

Arash Darafsheh; Consuelo Guardiola; Deepak Nihalani; Daeyeon Lee; Jarod C. Finlay; Alejandro Cárabe

doi:10.1117/12.2078019

11 March 2015 Biological super-resolution imaging by using novel microsphere-embedded coverslips

Arash Darafsheh, Consuelo Guardiola , Deepak Nihalani, Daeyeon Lee, Jarod C. Finlay, Alejandro Cárabe

Proceedings Volume 9337, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XII; 933705 (2015) https://doi.org/10.1117/12.2078019
Event: SPIE BiOS, 2015, San Francisco, California, United States

Abstract

Optical super-resolution imaging is a desirable technique in many fields, including medical and material sciences and nanophotonics. We demonstrated feasibility of optical microscopy, with resolution improvement by a factor of 2-3, by using microsphere-embedded coverslips composed of barium titanate glass microspheres (refractive index n~1.9-2.1) fixed in a transparent layer of polydimethylsiloxane. Imaging was performed by a conventional microscope and a fluorescent microscope with the microsphere-embedded layer placed in a contact position with various biological specimens and semiconductor nanostructures. We investigated a biomedical application of microsphere-assisted imaging technique by immunostaining of kidney sections where cellular distribution of a motor protein Myo1c and a podocyte specific protein ZO-1 was analyzed. A significant visual enhancement in the distribution pattern of the proteins was noted in the stained glomeruli by using microsphere-assisted imaging technique. Our results suggest that microsphere-assisted imaging technique is a promising candidate for applications in medical and cancer research, as well as in microfluidics and nanophotonics.

Citation Download Citation

Arash Darafsheh, Consuelo Guardiola , Deepak Nihalani, Daeyeon Lee, Jarod C. Finlay, and Alejandro Cárabe "Biological super-resolution imaging by using novel microsphere-embedded coverslips", Proc. SPIE 9337, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XII, 933705 (11 March 2015); https://doi.org/10.1117/12.2078019

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