Molecule-specific darkfield and multiphoton imaging using gold nanocages

Amy J. Powless; Samir V Jenkins; Mary Lee McKay; Jingyi Chen; Timothy J. Muldoon

doi:10.1117/12.2080152

12 March 2015 Molecule-specific darkfield and multiphoton imaging using gold nanocages

Amy J. Powless, Samir V Jenkins, Mary Lee McKay, Jingyi Chen, Timothy J. Muldoon

Proceedings Volume 9339, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications VII; 93390B (2015) https://doi.org/10.1117/12.2080152
Event: SPIE BiOS, 2015, San Francisco, California, United States

Abstract

Due to their robust optical properties, biological inertness, and readily adjustable surface chemistry, gold nanostructures have been demonstrated as contrast agents in a variety of biomedical imaging applications. One application is dynamic imaging of live cells using bioconjugated gold nanoparticles to monitor molecule trafficking mechanisms within cells; for instance, the regulatory pathway of epidermal growth factor receptor (EGFR) undergoing endocytosis. In this paper, we have demonstrated a method to track endocytosis of EGFR in MDA-MB-468 breast adenocarcinoma cells using bioconjugated gold nanocages (AuNCs) and multiphoton microscopy. Dynamic imaging was performed using a time series capture of 4 images every minute for one hour. Specific binding and internalization of the bioconjugated AuNCs was observed while the two control groups showed non-specific binding at fewer surface sites, leading to fewer bound AuNCs and no internalization.

Citation Download Citation

Amy J. Powless, Samir V Jenkins, Mary Lee McKay, Jingyi Chen, and Timothy J. Muldoon "Molecule-specific darkfield and multiphoton imaging using gold nanocages", Proc. SPIE 9339, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications VII, 93390B (12 March 2015); https://doi.org/10.1117/12.2080152

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available