Activatable thermo-sensitive ICG encapsulated pluronic nanocapsules for temperature sensitive fluorescence tomography

Tiffany C. Kwong; Farouk Nouizi; Uma Sampathkumaran; Yue Zhu; Maksudul M. Alam; Gultekin Gulsen

doi:10.1117/12.2080189

12 March 2015 Activatable thermo-sensitive ICG encapsulated pluronic nanocapsules for temperature sensitive fluorescence tomography

Tiffany C. Kwong, Farouk Nouizi, Uma Sampathkumaran, Yue Zhu, Maksudul M. Alam, Gultekin Gulsen

Author Affiliations +

Proceedings Volume 9339, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications VII; 93390C (2015) https://doi.org/10.1117/12.2080189
Event: SPIE BiOS, 2015, San Francisco, California, United States

Abstract

Fluorescent tomography has been hindered by poor tissue penetration and weak signal which results in poor spatial resolution and quantification accuracy. Recently, it has been reported that activatable temperature responsive fluorescent probes which respond to focused ultrasound heating can improve the resolution and quantification of fluorescent tomography in deep tissue. This has lead to a new imaging modality, "Temperature-modulated fluorescent tomography." This technique relies on activatable thermo-sensitive fluorescent nanocapsules for whose fluorescence quantum efficiency is temperature dependent. Within a 4-5° C temperature range, the fluorescent signal increase more than 10-fold. In this molecular probe, Indocyanine Green (ICG) is encapsulated inside the core of a thermo-reversible pluronic micelle. Here we show the fluorescence response and temperature range of the nanocapsules which have been optimized for a higher temperature range to be used for in vivo animal imaging. We report on the feasibility of these temperature-sensitive reversible nanocapsules for in vivo applications by studying the pharmacokinetics in a subcutaneous mouse tumor model in vivo.

Citation Download Citation

Tiffany C. Kwong, Farouk Nouizi, Uma Sampathkumaran, Yue Zhu, Maksudul M. Alam, and Gultekin Gulsen "Activatable thermo-sensitive ICG encapsulated pluronic nanocapsules for temperature sensitive fluorescence tomography", Proc. SPIE 9339, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications VII, 93390C (12 March 2015); https://doi.org/10.1117/12.2080189

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