Biomedical applications involving multiphoton probes

M. Potasek; K. Beeson; E. Parilov

doi:10.1117/12.2078363

12 March 2015 Biomedical applications involving multiphoton probes

M. Potasek, K. Beeson, E. Parilov

Proceedings Volume 9339, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications VII; 933913 (2015) https://doi.org/10.1117/12.2078363
Event: SPIE BiOS, 2015, San Francisco, California, United States

Abstract

Many techniques in biological and clinical science use multiphoton absorbers for fluorescence. The applications include medical imaging for living cells, diagnostic techniques for disease and spectroscopy. The intrinsic value of the multiphoton absorber coefficients is therefore of the utmost importance. Additionally, the laser intensity at which the absorber saturates can determine which absorber, dye or protein is useful for a particular application.

Yet, experimental methods for determining the optical coefficients often yield different results. We describe several common methods of 2PA measurements and describe their features. As an example of the importance of applying the correct analysis to measurements, we fit experimental data and obtain values for multiphoton absorbers and accurately obtain their intrinsic values. Finally, we present the optical properties of several multiphoton materials used in biology.

Citation Download Citation

M. Potasek, K. Beeson, and E. Parilov "Biomedical applications involving multiphoton probes", Proc. SPIE 9339, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications VII, 933913 (12 March 2015); https://doi.org/10.1117/12.2078363

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