Membrane-targeting peptides for nanoparticle-facilitated cellular imaging and analysis

Joyce Breger; James B. Delehanty III; Kelly Boeneman Gemmill; Lauren D. Field; Juan B. Blanco-Canosa; Philip E. Dawson; Alan L. Huston; Igor L. Medintz

doi:10.1117/12.2077026

12 March 2015 Membrane-targeting peptides for nanoparticle-facilitated cellular imaging and analysis

Joyce Breger, James B. Delehanty III, Kelly Boeneman Gemmill, Lauren D. Field, Juan B. Blanco-Canosa, Philip E. Dawson, Alan L. Huston, Igor L. Medintz

Author Affiliations +

Proceedings Volume 9338, Colloidal Nanoparticles for Biomedical Applications X; 93381P (2015) https://doi.org/10.1117/12.2077026
Event: SPIE BiOS, 2015, San Francisco, California, United States

Abstract

The controlled delivery of nanomaterials to the plasma membrane is critical for the development of nanoscale probes that can eventually enable cellular imaging and analysis of membrane processes. Chief among the requisite criteria are delivery/targeting modalities that result in the long-term residence (e.g., days) of the nanoparticles on the plasma membrane while simultaneously not interfering with regular cellular physiology and homeostasis. Our laboratory has developed a suite of peptidyl motifs that target semiconductor nanocrystals (quantum dots (QDs) to the plasma membrane where they remain resident for up to three days. Notably, only small a percentage of the QDs are endocytosed over this time course and cellular viability is maintained. This talk will highlight the utility of these peptide-QD constructs for cellular imaging and analysis.

Citation Download Citation

Joyce Breger, James B. Delehanty III, Kelly Boeneman Gemmill, Lauren D. Field, Juan B. Blanco-Canosa, Philip E. Dawson, Alan L. Huston, and Igor L. Medintz "Membrane-targeting peptides for nanoparticle-facilitated cellular imaging and analysis", Proc. SPIE 9338, Colloidal Nanoparticles for Biomedical Applications X, 93381P (12 March 2015); https://doi.org/10.1117/12.2077026

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available