Molecular effective coverage surface area of optical clearing agents for predicting optical clearing potential

Wei Feng; Ning Ma; Dan Zhu

doi:10.1117/12.2080643

13 March 2015 Molecular effective coverage surface area of optical clearing agents for predicting optical clearing potential

Wei Feng, Ning Ma, Dan Zhu

Author Affiliations +

Proceedings Volume 9322, Dynamics and Fluctuations in Biomedical Photonics XII; 932216 (2015) https://doi.org/10.1117/12.2080643
Event: SPIE BiOS, 2015, San Francisco, California, United States

Abstract

The improvement of methods for optical clearing agent prediction exerts an important impact on tissue optical clearing technique. The molecular dynamic simulation is one of the most convincing and simplest approaches to predict the optical clearing potential of agents by analyzing the hydrogen bonds, hydrogen bridges and hydrogen bridges type forming between agents and collagen. However, the above analysis methods still suffer from some problem such as analysis of cyclic molecule by reason of molecular conformation. In this study, a molecular effective coverage surface area based on the molecular dynamic simulation was proposed to predict the potential of optical clearing agents. Several typical cyclic molecules, fructose, glucose and chain molecules, sorbitol, xylitol were analyzed by calculating their molecular effective coverage surface area, hydrogen bonds, hydrogen bridges and hydrogen bridges type, respectively. In order to verify this analysis methods, in vitro skin samples optical clearing efficacy were measured after 25 min immersing in the solutions, fructose, glucose, sorbitol and xylitol at concentration of 3.5 M using 1951 USAF resolution test target. The experimental results show accordance with prediction of molecular effective coverage surface area. Further to compare molecular effective coverage surface area with other parameters, it can show that molecular effective coverage surface area has a better performance in predicting OCP of agents.

Citation Download Citation

Wei Feng, Ning Ma, and Dan Zhu "Molecular effective coverage surface area of optical clearing agents for predicting optical clearing potential", Proc. SPIE 9322, Dynamics and Fluctuations in Biomedical Photonics XII, 932216 (13 March 2015); https://doi.org/10.1117/12.2080643

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