Analysis of cell-tissue grafts under weightless conditions using confocal fluorescence microscopy

L. T. Volova; M. N. Milyakova; V. V. Rossinskaya; V. V. Boltovskaya; L. N. Kulagina; L. V. Kurganskaya; P. E. Timchenko; E. V. Timchenko; Larisa A Zherdeva Taskina

doi:10.1117/12.2179511

19 March 2015 Analysis of cell-tissue grafts under weightless conditions using confocal fluorescence microscopy

L. T. Volova, M. N. Milyakova, V. V. Rossinskaya, V. V. Boltovskaya, L. N. Kulagina, L. V. Kurganskaya, P. E. Timchenko, E. V. Timchenko, Larisa A Zherdeva Taskina

Author Affiliations +

Proceedings Volume 9448, Saratov Fall Meeting 2014: Optical Technologies in Biophysics and Medicine XVI; Laser Physics and Photonics XVI; and Computational Biophysics; 94480G (2015) https://doi.org/10.1117/12.2179511
Event: Saratov Fall Meeting 2014, 2014, Saratov, Russian Federation

Abstract

The research results of monitoring of viable cells in a cellular-tissue graft using confocal laser fluorescence microscopy at 488 nm and 561 nm with the use of fluorophore propidium iodide (propidium iodide, PI Sigma Aldrich USA) are presented. The processing of the received images was carried out using the software ANDOR. It is experimentally shown that the method of confocal fluorescence microscopy is one of the informational methods for detecting cells populated in a 3-D bio-carrier with a resolution of at least 400 nm. Analysis of the received micrographs suggests that the cells that were in a bio-carrier for 30 days in a synchronous ground-based experiment retained their viability compared to a similar space-based experiment in which the cells were hardly detected in a bio-carrier.

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L. T. Volova, M. N. Milyakova, V. V. Rossinskaya, V. V. Boltovskaya, L. N. Kulagina, L. V. Kurganskaya, P. E. Timchenko, E. V. Timchenko, and Larisa A Zherdeva Taskina "Analysis of cell-tissue grafts under weightless conditions using confocal fluorescence microscopy", Proc. SPIE 9448, Saratov Fall Meeting 2014: Optical Technologies in Biophysics and Medicine XVI; Laser Physics and Photonics XVI; and Computational Biophysics, 94480G (19 March 2015); https://doi.org/10.1117/12.2179511

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