The involvement of NF-κB in PDT-induced death of crayfish glial and nerve cells

E. V. Berezhnaya; M. A. Neginskaya; V. D. Kovaleva; M. V. Rudkovskii; A. B. Uzdensky

doi:10.1117/12.2179958

19 March 2015 The involvement of NF-κB in PDT-induced death of crayfish glial and nerve cells

E. V. Berezhnaya, M. A. Neginskaya, V. D. Kovaleva, M. V. Rudkovskii, A. B. Uzdensky

Proceedings Volume 9448, Saratov Fall Meeting 2014: Optical Technologies in Biophysics and Medicine XVI; Laser Physics and Photonics XVI; and Computational Biophysics; 94480N (2015) https://doi.org/10.1117/12.2179958
Event: Saratov Fall Meeting 2014, 2014, Saratov, Russian Federation

Abstract

Photodynamic therapy (PDT) is used for selective destruction of cells, in particular, for treatment of brain tumors. However, photodynamic treatment damages not only tumor cells, but also healthy neurons and glial cells. To study the possible role of NF-κB in photodynamic injury of neurons and glial cells, we investigated the combined effect of photodynamic treatment and NF-κB modulators: activator betulinic acid, or inhibitors parthenolide and CAPE on an isolated crayfish stretch receptor consisting of a single neuron surrounded by glial cells. A laser diode (670 nm, 0.4 W/cm2) was used as a light source. The inhibition of NF-κB during PDT increased the duration of neuron firing and glial necrosis and decreased neuron necrosis and glial apoptosis. The activation of NF-κB during PDT increased neuron necrosis and glial apoptosis and decreased glial necrosis. The difference between the effects of NF-κB modulators on photosensitized neurons and glial cells indicates the difference in NF-κB-mediated signaling pathways in these cell types. Thus, NF-κB is involved in PDT-induced shortening of neuron firing, neuronal and glial necrosis, and apoptosis of glial cells.

Citation Download Citation

E. V. Berezhnaya, M. A. Neginskaya, V. D. Kovaleva, M. V. Rudkovskii, and A. B. Uzdensky "The involvement of NF-κB in PDT-induced death of crayfish glial and nerve cells", Proc. SPIE 9448, Saratov Fall Meeting 2014: Optical Technologies in Biophysics and Medicine XVI; Laser Physics and Photonics XVI; and Computational Biophysics, 94480N (19 March 2015); https://doi.org/10.1117/12.2179958

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available