Quenching of photoexcited states of the proteins chromophores and introduced into the protein macromolecules fluorescent probes by heavy metal ions

A. G. Melnikov; O. A. Dyachuk; G. V. Melnikov

doi:10.1117/12.2178372

19 March 2015 Quenching of photoexcited states of the proteins chromophores and introduced into the protein macromolecules fluorescent probes by heavy metal ions

A. G. Melnikov, O. A. Dyachuk, G. V. Melnikov

Author Affiliations +

Proceedings Volume 9448, Saratov Fall Meeting 2014: Optical Technologies in Biophysics and Medicine XVI; Laser Physics and Photonics XVI; and Computational Biophysics; 94480S (2015) https://doi.org/10.1117/12.2178372
Event: Saratov Fall Meeting 2014, 2014, Saratov, Russian Federation

Abstract

We have studied the processes of quenching of photoexcited states of fluorescent probes and quenching of the fluorescence of the chromophores of human serum albumin (HSA) by heavy metal ions (HM): cations Tl⁺, Pb²⁺, Cu²⁺, Cd²⁺, and the anion of iodine (I^-). We used the dye from xanthene series – eosin as a fluorescent probe. By quenching of the fluorescence of protein chromophores we found an influence of HM on the structure of proteins, resulting in a shift of the peak of the fluorescence of HSA tryptophanyl. This can be explained by proteins denaturation under the influence of heavy metals and penetration of water into the inner environment of HSA tryptophan. It was established that the constant of the quenching of the probe phosphorescence is much higher than the fluorescence, which is explained by significantly longer lifetime of the photoexcited states of fluorescent probes in the triplet state than in the singlet.

Citation Download Citation

A. G. Melnikov, O. A. Dyachuk, and G. V. Melnikov "Quenching of photoexcited states of the proteins chromophores and introduced into the protein macromolecules fluorescent probes by heavy metal ions", Proc. SPIE 9448, Saratov Fall Meeting 2014: Optical Technologies in Biophysics and Medicine XVI; Laser Physics and Photonics XVI; and Computational Biophysics, 94480S (19 March 2015); https://doi.org/10.1117/12.2178372

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