Lens-free dark-field digital holographic microscopy for 3D tracking of microparticles

Oleg V. Grishin; Ivan V. Fedosov; Valery V. Tuchin

doi:10.1117/12.2179975

19 March 2015 Lens-free dark-field digital holographic microscopy for 3D tracking of microparticles

Oleg V. Grishin, Ivan V. Fedosov, Valery V. Tuchin

Proceedings Volume 9448, Saratov Fall Meeting 2014: Optical Technologies in Biophysics and Medicine XVI; Laser Physics and Photonics XVI; and Computational Biophysics; 944816 (2015) https://doi.org/10.1117/12.2179975
Event: Saratov Fall Meeting 2014, 2014, Saratov, Russian Federation

Abstract

We present a novel technique for 3D-tracking micro- and nanoparticles with original lens-free dark-field holographic microscope. Combining lens-free and dark-field microscopy principles this technique allows for high accuracy localization of micro- and nanoparticles using single hologram acquired with compact setup built with minimal use of optical components. In this paper, we present technique of particles localization, experimental setup, technique of digital correction of spherical aberration, results of simulation and experimental data.

Citation Download Citation

Oleg V. Grishin, Ivan V. Fedosov, and Valery V. Tuchin "Lens-free dark-field digital holographic microscopy for 3D tracking of microparticles", Proc. SPIE 9448, Saratov Fall Meeting 2014: Optical Technologies in Biophysics and Medicine XVI; Laser Physics and Photonics XVI; and Computational Biophysics, 944816 (19 March 2015); https://doi.org/10.1117/12.2179975

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